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nbp2 44607  (Novus Biologicals)


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    Novus Biologicals nbp2 44607
    Nbp2 44607, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nbp2 44607/product/Novus Biologicals
    Average 93 stars, based on 8 article reviews
    nbp2 44607 - by Bioz Stars, 2026-06
    93/100 stars

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    Fig. 2. Diosgenin regulated SIRT6 and reduced lipid accumulation in glomerulus. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus (B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and <t>WT1</t> (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus(D, n = 6 for each group); (E) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).
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    Antibodies and assay kits

    Journal: Kidney360

    Article Title: AMPK-SP1–Guided Dynein Expression Represents a New Energy-Responsive Mechanism and Therapeutic Target for Diabetic Nephropathy

    doi: 10.34067/KID.0000000000000392

    Figure Lengend Snippet: Antibodies and assay kits

    Article Snippet: Mouse anti-WT1 , Novus Biologicals , NBP2-44607.

    Techniques:

    Fig. 2. Diosgenin regulated SIRT6 and reduced lipid accumulation in glomerulus. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus (B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and WT1 (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus(D, n = 6 for each group); (E) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6.

    doi: 10.1016/j.phymed.2022.154276

    Figure Lengend Snippet: Fig. 2. Diosgenin regulated SIRT6 and reduced lipid accumulation in glomerulus. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus (B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and WT1 (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus(D, n = 6 for each group); (E) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).

    Article Snippet: The primary antibody includes SIRT6 (ab191385, abcam), WT1 (NBP2-44607,NOVUS), NEPHRIN Z. Wang et al. Phytomedicine 104 (2022) 154276 (ab216341, abcam), PODOCIN (ab181143, abcam), DESMIN (A3736, ABclonal).

    Techniques: Staining, Immunohistochemistry, Labeling, Immunofluorescence, Marker, Fluorescence, Quantitative RT-PCR

    Fig. 4. The effect of diosgenin in the regulation of SIRT6 and reduction of lipid accumulation in glomerulus was blocked by by OSS_128167 and MDL800 had the same effect as diosgenin. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus(B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and WT1 (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus (D, n = 6 for each group); (E) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6.

    doi: 10.1016/j.phymed.2022.154276

    Figure Lengend Snippet: Fig. 4. The effect of diosgenin in the regulation of SIRT6 and reduction of lipid accumulation in glomerulus was blocked by by OSS_128167 and MDL800 had the same effect as diosgenin. (A,B) SIRT6 stained by immunohistochemistry, scale bar, 25 μm (A) and mean optic intensity quantified by Image Pro Plus(B, n = 6 for each group); (C,D) Double labeling of immunofluorescence about SIRT6 and WT1 (marker of podocyte) by confocal, scale bar, 50 μm (C) and mean fluorescence intensity quantified by Image Pro Plus (D, n = 6 for each group); (E) Inflammation factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (F) Lipid metabolism factors in glomerulus were measured by RT-qPCR, n = 6 for each group; (G-I) Frozen section of kidney stained with Oil red and ADRP was stained by immunohistochemistry, scale bar, 25 μm (G), Oil red quantification by Image Pro Plus (H, n = 6 for each group) and ADRP quantified by Image Pro Plus (I, n = 6 for each group).

    Article Snippet: The primary antibody includes SIRT6 (ab191385, abcam), WT1 (NBP2-44607,NOVUS), NEPHRIN Z. Wang et al. Phytomedicine 104 (2022) 154276 (ab216341, abcam), PODOCIN (ab181143, abcam), DESMIN (A3736, ABclonal).

    Techniques: Staining, Immunohistochemistry, Labeling, Immunofluorescence, Marker, Fluorescence, Quantitative RT-PCR

    Fig. 5. Diosgenin protected against MPC5 injury by regulating SIRT6. (A-E) Protein including NEPHRIN and PODOCIN measured by western blot (A) and DESMIN was measured by immunofluorescence, scale bar, 50 μm (B) and NEPHRIN quantification (C, n = 6 for each group), PODOCIN quantification (D,n = 6 for each group) and DESMIN quantification (E,n = 6 for each group) by Image Pro Plus; (F-J) Protein including NEPHRIN and PODOCIN measured by western blot (F) and DESMIN was measured by immunofluorescence, scale bar, 50 μm (G) and NEPHRIN quantification (H, n = 6 for each group),PODOCIN quantification (I, n = 6 for each group) and DESMIN quantification (J, n = 6 for each group) by Image Pro Plus; (K and L) Double labeling of immunofluorescence about SIRT6 and WT1 by confocal,scale bar, 50 μm (K) and SIRT6 quantification by Image Pro Plus (L, n = 6 for each group).

    Journal: Phytomedicine : international journal of phytotherapy and phytopharmacology

    Article Title: Diosgenin protects against podocyte injury in early phase of diabetic nephropathy through regulating SIRT6.

    doi: 10.1016/j.phymed.2022.154276

    Figure Lengend Snippet: Fig. 5. Diosgenin protected against MPC5 injury by regulating SIRT6. (A-E) Protein including NEPHRIN and PODOCIN measured by western blot (A) and DESMIN was measured by immunofluorescence, scale bar, 50 μm (B) and NEPHRIN quantification (C, n = 6 for each group), PODOCIN quantification (D,n = 6 for each group) and DESMIN quantification (E,n = 6 for each group) by Image Pro Plus; (F-J) Protein including NEPHRIN and PODOCIN measured by western blot (F) and DESMIN was measured by immunofluorescence, scale bar, 50 μm (G) and NEPHRIN quantification (H, n = 6 for each group),PODOCIN quantification (I, n = 6 for each group) and DESMIN quantification (J, n = 6 for each group) by Image Pro Plus; (K and L) Double labeling of immunofluorescence about SIRT6 and WT1 by confocal,scale bar, 50 μm (K) and SIRT6 quantification by Image Pro Plus (L, n = 6 for each group).

    Article Snippet: The primary antibody includes SIRT6 (ab191385, abcam), WT1 (NBP2-44607,NOVUS), NEPHRIN Z. Wang et al. Phytomedicine 104 (2022) 154276 (ab216341, abcam), PODOCIN (ab181143, abcam), DESMIN (A3736, ABclonal).

    Techniques: Western Blot, Immunofluorescence, Labeling